TABLE 2.
Methods used to kill the Borrelia and the effect on frequency of coiling phagocytosis by human monocytes
| Tool | Application | Time | Subsequent washing | Coiling phagocytosis |
|---|---|---|---|---|
| Ethanol | 70% (vol/vol) | 15 min | Yes | Unaffected |
| Glutaraldehyde | 2.5% (vol/vol) | 15 min | Yesa | Unaffected |
| p-Formaldehyde | 4% (wt/vol) | 15 min | Yesa | Unaffected |
| Laboratory oven | 80°C | 5 min | No | Unaffected |
| 32-W UV bulb | 1-cm distance | 30 min | No | Unaffected |
| 30-W sonifier | 20 kHz | 15 s (twice) | No | Abolished |
a
Additional treatment with 1% (wt/vol) sodium borohydride for 10 min.