| Antibodies |
|
| Anti-Green Fluorescent Protein Chicken |
Aves Labs |
RRID: AB_10000240; Cat #: GFP-1020 |
| Alexa Fluor 488 Anti-Chicken |
Jackson Immuno |
RRID: AB_2340375; Cat #: 703–545-155 |
|
| Bacterial and virus strains |
|
| AAV9-hsyn-GRAB_ACh3.0 |
Vigene |
N/A |
| AAV5-Ef1a-DIO-eNpHR3.0-eYFP |
Gradinaru et al.58
|
Addgene, Cat #: 26966-AAV5 |
| AAV9- syn-FLEX-jGCaMP7f |
Dana et al.59
|
Addgene, Cat #: 104492-AAV9 |
| AAV1-mU6-Tac1r-gRNA1:EF1a-NucEnv-EGFP:hU6-Tacr1-gRNA2 |
This paper |
NIDA GEVVC Core, OTTC-VR-18–014 |
|
| Chemicals, peptides, and recombinant proteins |
|
| L-733,060 hydrochloride |
Tocris |
Cat #: 1145 |
| NASPM trihydrochloride |
Tocris |
Cat #: 2766 |
| Pirenzepine dihydrochloride |
Tocris |
Cat #: 1071 |
| Spermine tetrahydrochloride |
Tocris |
Cat #: S2876 |
| Picrotoxin |
Abcam |
Cat #: ab120315 |
| APV (DL-AP5) sodium salt |
Tocris |
Cat #: 3693 |
|
| Critical commercial assays |
|
| RNAscope multiplex fluorescent manual assay V1 |
ACD Bio |
Cat #: 320850 |
|
| Deposited data |
|
| Raw and analyzed data |
This paper |
DOI: 10.17605/OSF.IO/4FE3M
|
|
| Experimental models: Cell lines |
|
| Stellar competent cells |
Takara Inc |
Cat #: 636763 |
| NEB Stable competent cells |
New England Biolabs |
Cat #: C3040H |
|
| Experimental models: Organisms/strains |
|
| B6; 129S-Pdyntm1.1(cre)Mjkr/LowlJ (Dyn-Cre) |
Jackson Laboratories |
Stock #: 027958 |
| Tg(Adora2a-cre)KG139Gsat/Mmucd (A2a-Cre) |
Mutant Mouse Resource and Research Centers (MMRRC) |
Stock #: 031168-UCD |
| B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (Ai9) |
Jackson Laboratories |
Stock #: 007909 |
| C57BL/6J |
Jackson Laboratories |
Stock #: 000664 |
| B6; 129S6-Chattm2(cre)Lowl/J (ChAT-IRES-Cre) |
Jackson Laboratories |
Stock #: 006410 |
| B6J.129(B6N)-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh/J |
Jackson Laboratories |
Stock #: 026175 |
|
| Oligonucleotides |
|
| Mm-Tacr1-C3 (nucleotide target region 845–1775) |
ACD Bio |
GenBank #: NM_009313.5
|
| Mm-Chat-C2 (nucleotide target region 1090–1952) |
ACD Bio |
GenBank #: NM_009891.2
|
|
| Recombinant DNA |
|
| pAAV-hsyn-GRAB_ACh3.0 |
Jing et al.36
|
Addgene, Cat #: 121922 |
| pmU6-gRNA |
This paper |
Addgene, Cat #: 53187 |
| phU6-gRNA |
This paper |
Addgene, Cat #: 53188 |
| pOTTC1553 |
This paper |
Addgene, Cat #: 131682 |
| pOTTC1642 |
This paper |
Addgene, Cat #: 195018 |
|
| Software and algorithms |
|
| pClamp |
Molecular Devices |
Version 10 |
| Graphpad Prism |
Graphpad Software |
Version 8, 9, and 10 |
| Python custom script for extraction of calcium and acetylcholine events |
This paper |
https://doi.org/10.5281/zenodo.10048600
|
| Synapse |
Tucker-Davis Technologies |
N/A |
