TABLE 1.
hifA RFLP comparison of various H. influenzae clinical isolates by using AluI and RsaI
| hifA RFLP patterna | Strainb | Source and/or referencec | Capsular type |
|---|---|---|---|
| 1 | Eagan (E1a) | CSF (10) | b |
| AAr108 | Throat (13) | b | |
| AA61 | Blood (13) | b | |
| R9 | (13) | b | |
| AAr120 | Trachea (this study) | f | |
| AAr39 | Throat (11) | NTf | |
| AAr176d | Nasopharynx (11) | NT | |
| AAr64 | Trachea (13) | b (lost capsule) | |
| AAr169 | Sinus (11) | NT | |
| AAr117 | Nasopharynx (this study) | b (lost capsule) | |
| 2 | Mr31 | Middle ear (11) | NT |
| AAr49d | Throat (11) | NT | |
| 1712MEE (LB5)d | Middle ear (11) | NT | |
| AAr91 | Throat (11) | NT | |
| 3 | M96 | CSF (this study) | a |
| M43e | Throat (12) | b | |
| AM30 (770235) | CSF (39) | b | |
| MinnA | CSF (3) | b | |
| AO2 | Throat (22) | b | |
| AAr122 | Nasopharynx (13) | b | |
| SL2 | Throat (13) | b | |
| AAr7 | Nasopharynx (23) | b | |
| AAr103 | Eye (23) | b | |
| AAr119 | Nasopharynx (13) | b | |
| ATCC 9007d | ATCC | c | |
| AAr45 | Nasopharynx (11) | NT | |
| AAr154 | Nasopharynx (11) | NT | |
| AAr60 | Peritonsillar abscess (this study) | NT | |
| 86-1249 | Middle ear (1) | NT | |
| 4 | ATCC 9008 | Throat (ATCC) | d |
| AAr191 | Throat (this study) | e | |
| AAr73d | Nasopharynx (11) | NT | |
| 86-042NP (LB1) | Nasopharynx (11) | NT | |
| 1128MEE (LB2) | Middle ear (11) | NT | |
| AAr160d | Trachea (11) | NT | |
| 5 | AAr107 | Nasopharynx (this study) | e |
| AA18d | Blood (this study) | f | |
| AAr9 | Throat (this study) | f | |
| AAr32d | Throat (this study) | f | |
| AAr80 | Nasopharynx (this study) | f | |
| AA12 | Blood (this study) | f | |
| AA58 | Blood (this study) | f | |
| AA195 | Blood (this study) | f | |
| 6 | ATCC 9006d | ATCC | a |
| AAr157d | Nasopharynx (11) | NT | |
| AAr180 | Sputum (11) | NT | |
| 7 | 86-0295 | Middle ear (1) | NT |
| 8 | 81-0384 | Middle ear (1) | NT |
| 9 | M37 | Nasopharynx (3) | NT |
| 10 | F3037 | Blood (37) | NT |
| F3031 | Blood (43) | NT |
hifA RFLP fragment sizes (in base pairs) based on DNA sequences. Pattern 1: AluI, 374, 213, 41, 23; RsaI, 411, 117, 86, 37. Pattern 2: AluI, 288, 243, 41, 29, 23; RsaI, 243, 189, 155, 37. Pattern 3: AluI, 365, 159, 95, 23; RsaI, 264, 144, 111, 68, 37, 18. Pattern 4: AluI, 320, 112, 80, 48, 41, 23; RsaI, 501, 86, 37. Pattern 5: AluI, 314, 235, 55, 23, 10; RsaI, 263, 254, 83, 37. Pattern 6: AluI, 262, 146, 100, 54, 41, 23; RsaI, 589, 37. Pattern 7: AluI, 272, 127, 98, 60, 47, 23, 12; RsaI, 339, 228, 72. Pattern 8: AluI, 613, 23; RsaI, 264, 228, 84, 48, 12. Pattern 9: AluI, 380, 236, 23; RsaI, 335, 267, 37. Pattern 10: AluI, 284, 165, 164, 23; RsaI, none.
Strains with published hifA sequences are in boldface type. Database accession numbers or references are as follows: Eagan (E1a), M64334; M43, reference 12; AO2, X52419; AM30 (770235), X16991; 86-1249, U19761; 86-0295, U19730; 81-0384, U19795; M37 and MinnA, reference 3; F3037, S59288; F3031, L08606.
CSF, cerebrospinal fluid; ATCC, American Type Culture Collection.
H. influenzae strains whose hifA genes were sequenced in this study.
The hifA sequences from strains M43, AM30 (770235), and MinnA are identical and are treated as one sequence throughout this article.
NT, nontypeable H. influenzae.