TABLE 1.

Primers used in this study^a

Primer	Sequence (5′-3′)	Gene	Position
PF	TGTCTCTGTCACGCATTGTC	prn	152–171
AF	GCCAATGTCACGGTCCAA	prn	649–666
AR	GCAAGGTGATCGACAGGG	prn	1234–1217
BR	CGGATTCAGGCGCAACTC	prn	2076–2059
BF	AGCTGGGCGGTTCAAGGT	prn	1542–1559
PR	ATGCCGTTGGTGTGTACCGT	prn	2714–2695
S1F	TAGGCACCATCAAAACGCAG	s1	474–493
S1FM	ACAATGCCGGCCGTATCCTC	s1	946–965
S1R	TCAATTACCGGAGTTGGGCG	s1	1350–1330

^aThe gene harboring the target sequence (italicized) is indicated. A schematic representation of primer positions in the prn gene is depicted in Fig. 1. Primers were derived from published sequences of prn (8) and the S1 subunit (20, 24).