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. Author manuscript; available in PMC: 2024 Jan 18.
Published in final edited form as: Cell Rep Phys Sci. 2023 Jul 19;4(7):101459. doi: 10.1016/j.xcrp.2023.101459

Table 1.

Amino acid sequence, theoretical values of normalized hydrophobicity, normalized hydrophobic moment, net charge, ratio of polar/non-polar amino acid residues, propensity to in vitro aggregation, amphiphilicity index, and values of helical fraction in water and in a mixture 3:2 of TFE and water, for WT1, WT2, and their corresponding Ala-Scan analogs

Peptides
 Physicochemical properties
 Helical fraction
Code Sequence <H> <μH> q P/N In vitro aggregation Amphiphilicity index fH (water) fH (TFE:water)
WT1 LKLMGIVKKVLGAL −0.39 0.40 4 0.56 15.42 0.79 0.05 0.34
A1–1 AKLMGIVKKVLGAL −0.36 0.42 4 0.56 15.37 0.79 0.05 0.32
A2–1 LALMGIVKKVLGAL −0.54 0.33 3 0.40 27.36 0.52 0.04 0.29
A3–1 LKAMGIVKKVLGAL −0.36 0.37 4 0.56 13.64 0.79 0.07 0.29
A4–1 LKLAGIVKKVLGAL −0.39 0.40 4 0.56 14.93 0.79 0.05 0.38
A5–1 LKLMAIVKKVLGAL −0.40 0.39 4 0.40 29.98 0.79 0.09 0.44
A6–1 LKLMGAVKKVLGAL −0.34 0.40 4 0.56 13.64 0.79 0.05 0.33
A7–1 LKLMGIAKKVLGAL −0.36 0.37 4 0.56 13.65 0.79 0.09 0.31
A8–1 LKLMGIVAKVLGAL −0.54 0.37 3 0.40 34.40 0.52 0.08 0.24
A9–1 LKLMGIVKAVLGAL −0.54 0.29 3 0.40 56.15 0.52 0.11 0.22
A10–1 LKLMGIVKKALGAL −0.36 0.38 4 0.56 2.75 0.79 0.09 0.23
A11–1 LKLMGIVKKVAGAL −0.36 0.38 4 0.56 3.86 0.79 0.07 0.16
A12–1 LKLMGIVKKVLAAL −0.40 0.39 4 0.40 102.82 0.79 0.13 0.35
A14–1 LKLMGIVKKVLGAA −0.36 0.37 4 0.56 3.84 0.79 0.05 0.25
WT2 LKLLGIVKKVLGAI −0.45 0.43 4 0.56 34.76 0.79 0.10 0.23
A1–2 AKLLGIVKKVLGAI −0.41 0.45 4 0.56 34.49 0.79 0.10 0.33
A2–2 LALLGIVKKVLGAI −0.60 0.35 3 0.40 85.50 0.52 0.15 0.29
A3–2 LKALGIVKKVLGAI −0.41 0.41 4 0.56 27.74 0.79 0.06 0.26
A4–2 LKLAGIVKKVLGAI −0.41 0.42 4 0.56 27.89 0.79 0.08 0.31
A5–2 LKLLAIVKKVLGAI −0.46 0.42 4 0.40 94.76 0.79 0.10 0.30
A6–2 LKLLGAVKKVLGAI −0.39 0.44 4 0.56 27.13 0.79 0.10 0.24
A7–2 LKLLGIAKKVLGAI −0.41 0.40 4 0.56 27.10 0.79 0.08 0.24
A8–2 LKLLGIVAKVLGAI −0.60 0.41 3 0.40 83.03 0.52 0.06 0.29
A9–2 LKLLGIVKAVLGAI −0.60 0.31 3 0.40 108.91 0.52 0.06 0.31
A10–2 LKLLGIVKKALGAI −0.41 0.41 4 0.56 10.16 0.79 0.06 0.31
A11–2 LKLLGIVKKVAGAI −0.41 0.41 4 0.56 12.38 0.79 0.11 0.25
A12–2 LKLLGIVKKVLAAI −0.46 0.43 4 0.40 177.88 0.79 0.08 0.38
A14–2 LKLLGIVKKVLGAA −0.39 0.38 4 0.56 10.31 0.79 0.07 0.24

<H>, normalized hydrophobicity; <μH>, normalized hydrophobic moment; q, net charge; P/N, ratio of polar/non-polar amino acid residues; fH, helical fraction.