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. Author manuscript; available in PMC: 2024 Jan 18.
Published in final edited form as: Cell Rep Phys Sci. 2023 Jul 19;4(7):101459. doi: 10.1016/j.xcrp.2023.101459

Table 2.

Properties of peptides WT1, WT2, and their corresponding second-generation analogs

Peptides
 Physicochemical properties
 Helical fraction
Code Sequence <H> <μH> q P/N In vitro aggregation Amphiphilicity index fH (water) fH (TFE:water)
WT1 LKLMGIVKKVLGAL −0.39 0.40 4 0.56 15.42 0.79 0.05 0.34
I14–1 LKLMGIVKKVLGAI −0.42 0.42 4 0.56 28.33 0.79 0.19 0.41
L4–1 LKLLGIVKKVLGAL −0.42 0.41 4 0.56 21.87 0.79 0.14 0.88
K12–1 LKLMGIVKKVLKAL −0.25 0.53 5 0.56 1.76 1.05 0.06 0.62
K13–1 LKLMGIVKKVLGKL −0.24 0.47 5 0.75 1.77 1.05 0.14 0.13
WT2 LKLLGIVKKVLGAI −0.45 0.43 4 0.56 34.76 0.79 0.10 0.23
K1–2 KKLLGIVKKVLGAI −0.26 0.57 5 0.75 31.51 1.05 0.12 0.62
K6–2 LKLLGKVKKVLGAI −0.24 0.48 5 0.75 26.61 1.05 0.10 0.41
K10–2 LKLLGIVKKKLGAI −0.26 0.35 5 0.75 8.22 1.05 0.14 0.50
K13–2 LKLLGIVKKVLGKI −0.29 0.51 5 0.75 8.15 1.05 0.15 0.89

Theoretical values of: normalized hydrophobicity (<H>); normalized hydrophobic moment (<μH>); net charge (q); ratio of polar/non-polar amino acid residues (P/N); propensity to aggregate in vitro; amphiphilicity index; and values of helical fraction (fH) in water and in a mixture 3:2 of TFE and water.