Zeta potential for TC-CS-SP NPs prepared at optimal CS to SP molar ratios (1 : 1) incubated at different phosphate bufsfer (pH 6, pH 7.4) at different temperature (mean ± SD, n = 3).

Zeta potential (mV)
Temperature	37 °C		4 °C
Time (h)	pH 7.4	pH 6	pH 7.4	pH 6
24	−1.6 ± 0.6	0.4 ± 0.3	−1.6 ± 0.4	0.8 ± 0.5
48	−2.3 ± 0.1	−0.1 ± 0.5	−2.7 ± 0.9	0.7 ± 0.4
168	−3.8 ± 0.4	1.5 ± 0.6	−0.6 ± 0.4	0.8 ± 0.4