TABLE 1.
IP response to T. denticola ATCC 35405 and OM extract
| Assay sample | Relative cpm in [3H]IPsa
|
% Change, 0–60 min | |
|---|---|---|---|
| 0 min | 60 min | ||
| 1% FBS (bacterium-free control) | 100 | 254 | +154 |
| +Whole 35405 | 94 | 169 | +79 |
| +Whole 35405/PMSFc | 120 | 170 | +42 |
| +Whole 35405/TLCKc | 100 | 177 | +77 |
| 15% FBS (bacterium-free control) | 123 | 459 | +273 |
| 1% FBS + ATP (bacterium-free control)b | 100 | 382 | +282 |
| +Whole 35405 | 132 | 347 | +163 |
| 1% FBS (OM-free control) | 100 | 161 | +61 |
| +1/4 OMd | 117 | 119 | +2 |
| +1/4 OM/PMSFc | 81 | 92 | +11 |
| +1/8 OM | 96 | 128 | +33 |
| +1/8 OM/PMSF | 75 | 94 | +25 |
| +1/16 OM | 113 | 209 | +85 |
| +1/16 OM/PMSF | 94 | 207 | +120 |
| 15% FBS (OM-free control) | 100 | 307 | +207 |
a
Value relative to 1.0% FBS–LiCl background control at beginning of experiment, time 0 min (100%); average of duplicates.
b
HGF were pretreated with T. denticola or untreated (control) for 30 min prior to addition of ATP. Data are for 0 and 60 min after addition of ATP.
c
T. denticola cells or OM extract were pretreated with PMSF at either 170 μg/ml to inhibit chymotrypsin-like or TLCK at 150 μg/ml to inhibit trypsin-like proteinase activity.
d
1/4 dilution of T. denticola ATCC 35405 OM extract.