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. Author manuscript; available in PMC: 2024 Jan 18.
Published in final edited form as: Dev Cell. 2021 Oct 4;56(19):2752–2764.e6. doi: 10.1016/j.devcel.2021.09.014

Figure 3. SK-N-AS cells in the developing murine head undergo neuronal differentiation.

Figure 3.

(A) Lateral view schematic of a mouse E10.5 embryo indicating the locations of head and trunk xenotransplantation.

(B–E′)5 μm optical slices of whole-mount immunolabeling for anti-neurofilament (NF; white) of representative SK-N-AS NB (B–C′) or A375 melanoma (D–E′) cells at 14 hpi in E10.5 mouse embryos. Head injections targeted a region populated by cranial neural crest cells, analogous to that targeted in zebrafish. 71% of assayed embryos (n = 12/17 embryos) with SK-N-AS injections into the head harbored NF-positive cells, whereas 0% of assayed embryos (n = 0/11 embryos) with SK-N-AS injections into the trunk harbored NF-positive cells. No embryos with A375 melanoma injections into the head (n = 0/16 embryos) or trunk (n = 0/14 embryos) harbored NF-positive cells. Scale bar, 10 μm.