Figure 4.
Recurrent severe protein depletion extends the lifespan of tumor-bearing flies. (A-C) Lifespans of RAFgof expressing flies on various feeding regimes at 29°C, with details of each regime depicted below the plots. Regime 1 (A) and Regime 2 (B) only vary in the duration of the initial PDD phase. Regime 3 (C) starts with CD instead of PDD and had shorter alternating PDD and longer CD phases. All experiments were conducted simultaneously, and data are superimposed onto the data shown in Fig. 3A (dashed lines, this data was collected in an independent trial). (D) Maximum lifespans of the 10% longest-living flies. (E) Table summarizing median and maximum (10%) lifespans of all tested groups. (F) Control lifespan experiment of healthy flies subjected to Regime 2 or CD. All flies were kept at 29°C with n=360-450 flies per treatment.