Figure 5.

Recurrent protein depletion rescues tumor phenotype. (A) Sections through thorax (upper panel) or abdomen (lower panel) of control and RAF^gof expressing flies fed on CD and on Regime 2. The GFP⁺ esg⁺ cells attached to the basal membrane (arrowheads) and protruding mass (asterisk) visible in RAF^gof expressing flies subjected to CD are mostly absent following Regime 2 feeding. That feeding also resulted in detached esg⁺ cells (yellow arrowhead, scale bar: 50 µm). Images were taken after 10 days of treatment. (B) Co-expressed luciferase in progenitor cells in control flies on CD and RAF^gof expressing flies on Regime 1 feeding for 12 to 18 days (n=5-10). (C) Total gut area per cross section, (D) lumen volume per cross section and (E) lumen-to-gut ratio of control and RAF^gof expressing flies subjected to CD or Regime 2 feeding for 10 days (n=9-11). (F) Fecal spot size and (G) fecal spot numbers at day 10 (n=7-21). (H) Basic metabolic rate at day 10 (n=5-10).