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. 2023 Nov 9;12(2):2276337. doi: 10.1080/22221751.2023.2276337

Figure 2.

Figure 2.

Schematic and screening of crRNA and primers for the detection of HDV RNA. A. Location of the crRNA and RT–PCR primer sets for the RT–PCR-CRISPR assay. B. Location of the crRNA and RT-RAA primer sets for the RT-RAA-CRISPR assay. C. Kinetics of fluorescence signal within 60 min and fluorescence values in 30 min of four different crRNAs for RT–PCR-CRISPR reaction. D. Kinetics of the fluorescence signal within 60 min and fluorescence values in 30 min of four different crRNAs for the RT-RAA-CRISPR reaction. E. Heatmaps of 9 different primer pairs for RT–PCR-CRISPR reaction. F. Heatmaps of 9 different primer pairs for the RT-RAA-CRISPR reaction. Forward denoted by “F”, reverse denoted by “R”. The data are the mean ± s.d. for 3 technical replicates. NC, Negative controls.