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. 2023 Nov 9;12(2):2276337. doi: 10.1080/22221751.2023.2276337

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 5. — Evaluation of RT–PCR-CRISPR and RT-RAA-CRISPR assays using HDV-positive samples. A. Fluorescence values of RT–PCR-CRISPR for HDV-positive sample serial dilutions. B. Fluorescence values of RT-RAA-CRISPR for HDV-positive sample dilutions. C. Ct values of RT–qPCR for HDV-positive sample serial dilutions. D. Scatter plot of ddPCR for HDV-positive sample serial dilutions. E. Fluorescence values of RT–PCR-CRISPR for different positive samples (HCV, HEV, HIV, HBV RNA). F. Fluorescence values of RT-RAA-CRISPR for different positive samples (HCV, HEV, HIV, HBV RNA). The data are the mean ± s.d. for 3 technical replicates. NC, Negative controls. *: p < 0.05, **: p < 0.01, ***: p < 0.001.