Figure S4. Characterization of the SNARE complex assembly.
(A) Representative immunoblot showing the presence of the SNARE complex when v-lipos are treated with t-lipo; the antibody used was SNAP-25B, as indicated. n = 3 independent sets of experiments, three sets of liposomes. (B) Representative immunoblot showing the presence and absence of the SNARE complex, before and after heating the reaction mixtures at 95°C, respectively. Here, ND5S were treated with t-SNARE liposomes; the antibody used was SNAP-25B, as indicated. n = 3 independent sets of experiments. (C) Representative immunoblot showing the absence and presence of the SNARE complex, after and before heating the reaction mixtures at 95°C, respectively. Here, SVs were treated with t-SNARE liposomes; the antibody used was syb2, as indicated. n = 3 independent sets of experiments.
Source data are available for this figure.
