Fig. 2.

CTCF binds to two sites in the ESR1 gene. a ESR1 genomic domains showing the TSSs of the promoters and first exon. b ESR1 genomic domains showing the putative CTSs as yellow triangles and the CpG sites as black vertical lines. The many CpG sites (~ -597 to + 955) in ESR1 show the presence of an HCI, which is susceptible to DNA methylation. c CTCF ChIP in MCF-7 and MDA-MB-231 cells at CTS1 and CTS2. H3K4ac ChIP was performed in the same loci to evaluate for heterochromatin and euchromatin