Fig. 1.

LAIR-1 triggering inhibits T cell effector functions. A Pan T cells were isolated from spleen and stimulated with indicated concentrations of immobilized anti-CD3 (clone 145-2C11), in the presence of an isotype control (blue) or an agonistic LAIR-1 antibody (clone 113, red) and proliferation was assessed after 3 days by flow cytometry for both CD4⁺ (left) and CD8⁺ (right) T cells. B As in (A), but activation status of T cells, as determined by CD25 and CD69 surface expression, was assessed by flow cytometry. C Supernatant of cultures as in (A) was harvested and cytokine production of T cell cultures was assessed by ELISA. Graphs depict three mice from one representative experiment. Statistical significance was assessed by two-way ANOVA, for Granzyme B significance is indicated for overall effect of LAIR-1 ligation. P values below 0.1 are depicted