Fig. 3.

Mouse LAIR-1 signals through a wider array of synthetic collagen peptides and is blocked to a lesser extent than human LAIR-1. A, B The synthetic peptides of the human collagen II Toolkit (A) and collagen III Toolkit (B) were immobilized and mouse or human LAIR-1-CD3ζ reporter cells were applied, then LAIR-1 signalling as determined by GFP production was assessed. CRP = Collagen Related Peptide, GPP = peptide consisting of 10 glycine-proline-proline repeats, BSA = bovine serum albumin. Col2 and Col3 indicate immobilized full-length collagen. Human LAIR-1-CD3ζ NFAT-GFP reporter data have been published previously [38]. C Human and mouse LAIR-1-CD3ζ NFAT-GFP reporter lines were stimulated with immobilized human and mouse collagen I, and NC410 was titrated to block LAIR-1:collagen binding. Data are aggregated from 3 separately performed experiments with 2–3 technical replicates for each condition. Significance was assessed using a two-way ANOVA