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. 2024 Jan 18;15(1):66. doi: 10.1038/s41419-024-06455-6

Fig. 2. Sp1 regulates GSDME expression.

Fig. 2

A, C, and E HeLa cells transfected by the control or Sp1 shRNA or treated with the indicated amounts of inhibitors, plicamycin or peretinoin, were harvested and lysed in the RIPA lysis buffer. Western blotting analysis of lysates from the cells shows the Sp1, GSDME, and β-actin levels. B, D, and F HeLa cells transfected by the control or Sp1 shRNA, or treated with the indicated amounts of inhibitors plicamycin or peretinoin. qPCR analysis shows GSDME mRNA level, normalized by β-actin level. G qPCR analysis result of GSDME mRNA level in HeLa cells transfected by indicated amounts of Sp1 plasmids.