| The primers | 5′-TGCAAGGGAAGCTCACAGTC-3′ (forward) |
| 5′-GATTGGTGGCACCTC TTAATG-3′ (reverse) | |
| GAPDH | CCACTC CTC CACCTTTGAC (forward) |
| ACCCTGTTGCTGTAGCCA (reverse) | |
| The probe sequence | GCGCTGGCGCGGGGCTGTGTCTAC [C/T] |
| CTTCAGTAAGTGGTCAAAACCTG [GC] |
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