FIG. 2.

Southern blot analysis of transgenic mice produced by injection of the Q9 gene in (C57BL/6J × BALB/cAnN)F₁ embryos. (A) Map of microinjected Q9 gene. Relevant restriction sites: B, BamHI; A, ApaI; E, EcoRI. The JBS9 probe is a 500-bp BamHI-SalI fragment corresponding to a segment of the large intron between exons 3 and 4 (16) which hybridizes with a 2.7-kb BamHI fragment. (B) Genomic DNA isolated from tail biopsies was digested with BamHI and hybridized with the JBS9 probe. Lane 1, DNA from a Q9 transgenic Tg1 male mouse; lane 2, DNA from a Q9 transgenic Tg2 male mouse; lane 3, DNA from a nontransgenic littermate mouse; lane 4, DNA from a C57BL6/J mouse.