Figure 1.

Scheme of the LORE1 mutant screening to identify lines affected in the intercellular infection by IRBG74. Approximately 200,000 LORE1 plants were inoculated with IRBG74, selecting 1,200 mutants with a Nod- or Fix- phenotype at 6-8 wpi. The offspring seeds of 571 selected lines were inoculated on plates (2^nd screening), confirming the nodulation phenotype in 110 lines. For this study, we conducted the FST poolit method to identify the LORE1 insertions sites in the genome of 47 selected mutants.