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. 2024 Jan 18;10:36. doi: 10.1038/s41420-024-01798-7

Fig. 4. PAK4 knockdown chemosensitized NSCLC cells via modulating ER stress.

Fig. 4

A PAK4 and GRP78 expression profiles were examined by western blot analysis in A549/A549-res and NCI-H520/NCI-H520-res cells. B A549-res and NCI-H520-res cells stably expressing PAK4 shRNA (shPAK4) or control shRNA (shCtrl) were treated with 5 µmol/L cisplatin for 48 h. PAK4 and GRP78 expression was examined by western blot analysis (left panel) and Western blot analyses of PAK4 and GRP78 expression in PAK4 overexpressed cells (right panel). C A549-res and NCI-H520-res cells stably expressing PAK4 shRNA (shPAK4) or control shRNA (shCtrl) or shPAK4 and GRP78 plasmids were treated with 5 µmol/L cisplatin for 48 h. Cytoplasmic calcium level was examined by flow cytometry assay. D A549-res and NCI-H520-res cells stably expressing PAK4 shRNA (shPAK4) or control shRNA (shCtrl) were transfected with GRP78 or control vector, followed by treatment with 5 µmol/L cisplatin for 48 h. Apoptosis rates were determined by flow cytometry. Data are shown as the means of three independent experiments or representative data. Data are expressed as mean ± standard deviation. **P < 0.01, ***P < 0.001 by Student’s t test.