FIG. 9.
Protective effect of gp43 and P10 immunization in intratracheally infected mice (3 × 105 virulent P. brasiliensis yeast forms). (A) Lung section from a control nonimmunized BALB/c mouse showing confluent epithelioid granulomata forming a paracoccidioma-like lesion with a great number of viable yeast cells. (B) Dissemination to the liver of P. brasiliensis in a control nonimmunized mouse; confluent epithelioid granulomas with fungal cells are shown. (C) A single, well-demarcated epithelioid granuloma, with a prominent mononuclear cellular halo, encircling viable and nonviable yeast forms in a lung section from a gp43-immunized animal. (D) Apparently resolved granuloma, containing few macrophages and no fungal cells, in a lung section of a P10-immunized mouse.