TABLE 1.
Cytokine detection in the supernatant fluids of lymphocyte cultures from sensitized BALB/c micea
| Immunogen | Cytokine content (pg/ml)
|
|||||||
|---|---|---|---|---|---|---|---|---|
| IL-2b | IL-4 | IL-5 | IL-6 | IL-9 | IL-10 | IL-12 | IFN-γc | |
| gp43 | 1,615 | −d | − | − | − | − | − | 390 |
| P10 | 3,501 | − | − | − | − | − | − | 450 |
| CFA only | 300 | − | − | − | − | − | − | − |
Similar results were obtained with the A/Sn mouse lineage.
IL-2 content (in counts per minute) determined by methyl-[3H]thymidine incorporation by CTLL cells; 24-h culture supernatants were used. Negative controls and counts were as follows: culture medium only, 117 cpm; with monoclonal anti-IL-2 antibody, 74 cpm (gp43) and 100 cpm (P10). The positive control (30 IU of rIL-2/ml) had 7,144 cpm.
IFN-γ contents in 6-day culture supernatants are the averages of two determinations and were calculated from a standard curve for rIFN-γ (Pharmingen) (105 U/10 μg). With 4 × 106 cells/ml, IFN-γ was only produced in the presence of 30 IU of IL-2/ml.
−, not detectable by the ELISA method.