TABLE 3.
Anti-gp43 antibodies in sera from BALB/c mice immunized with gp43 and P10 and infected with 3 × 105 yeast forms of P. brasiliensis
| Immunizationa | Infection time (mo) | ELISA titerb | Immunoglobulins | IDe for gp43 |
|---|---|---|---|---|
| Nonimmunized | 1 | 1:800 | BRc | Negative |
| gp43 | 1 | 1:409,600 | IgG1 > IgE > Ig2a > IgG2b > IgM > IgG3 = IgA | Positive |
| P10 | 1 | 1:200 | BR | Negative |
| Nonimmunized | 3.5 | 1:51,200 | IgG1 = IgG2a > IgE > IgG2b | Positive |
| gp43 | 3.5 | 1:409,600 | IgG1 > IgG2a > IgG2b > IgE > IgM > IgG3 | Positive |
| P10 | 3.5 | 1:3,200 | IgG1d | Negative |
a
As described in Materials and Methods.
b
Cutoff for the ELISAs was set at A492 = 0.100.
c
BR, background reactivity (A492 = 0.100) at the middle of the titration curve.
d
Very small amounts of anti-gp43 IgG1 detected.
e
ID, double immunodiffusion test with gp43 at 1 μg/well.