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Recovery and viability of macrophages after culture with M. tuberculosis. Monocyte-derived macrophages (4 × 105 per well) were cultured for 10 days in the presence of media alone or with H37Ra, H37Rv, or clinical isolate AS. The numbers of macrophages recovered and their percent viabilities, as assessed by Trypan blue exclusion, were determined. The values shown are the means and standard errors for four experiments.
