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. 2023 Nov 17;11(3):2304017. doi: 10.1002/advs.202304017

Figure 2.

Figure 2

Developmental trajectory of middle layer.A) UMAP projections showing middle layer cells populations. ML1 to ML3, sub‐cell clusters. B) UMAP of the pseudotime trajectory of middle layer cells by using Monocle3. C,D) Simulation of the successive developmental trajectory of middle layer over pseudo‐time by using Monocle2. E) Expression patterns of TGA10, ARPN, TCH4, and CYSB over pseudo‐time. F) Heatmap showing hierarchical clustering of the expression of DEGs in each pseudotime cluster. GO terms and p value of each gene cluster are shown in the table on the right. G) Observation of the middle layer in cotton anther at stage 7 to stage 11. Scale bar, 20 µm. TGA10, TGACG MOTIF‐BINDING PROTEIN 10; ARPN, PLANTACYANIN; TCH4, TOUCH 4; CYSB, CYSTATIN B; ML, middle layer; T, tapetum.