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. 2023 Nov 20;11(3):2304926. doi: 10.1002/advs.202304926

Figure 4.

Figure 4

KRASG12D eSimoa assay in the luminal eSimoa pipeline. a) The standard curve of the KRASG12D Simoa assay. The LOD value of the assay was 3.18 pg mL−1 (151 fm). b,c) Validation of the specificity of KRASG12D Simoa assay using a cross‐testing method. KRASG12D proteins were measured by pan‐RAS eSimoa assay. RAS proteins were measured by KRASG12D eSimoa assay, which showed no detection of wildtype RAS protein even at high concentrations. d,e) KRASG12D protein levels quantified in different concentrations of lysed PANC‐1 EVs, HepG2 EVs, and the EV standard. The LOD values were 5.8 × 107 EV mL−1 for PANC‐1 EVs and 8.1 × 108 EV mL−1 for HepG2 EVs. No detectable KRASG12D protein was observed for the EV standard, which was as expected. f) KRASG12D signals were measured in SEC fractions of PANC‐1 EVs, which showed that KRASG12D proteins were only present in the EV fractions. All measurements were performed in duplicate. The dotted lines locate the LOD of the assay.