Figure 7.

Impact of deformation on hydrogel rheology and triple‐helical self‐assembly. A) Altered temperature denaturation profile of a previously deformed 1.0% w/v hydrogel returns to its original form following annealing. The temperature response was monitored at 1% strain and 3 rad/s. Closed (⬤) and open circles (○) report storage and loss moduli. B) Extrusion of a 0.5% w/v hydrogel produces a white precipitate and reveals a second melting transition in CD thermal denaturation experiments, which is in agreement with rheology data. CD spectra and visual inspection reveal the restoration of the original sample upon annealing. The signal was monitored at 226 nm in all denaturation experiments. The initial denaturation curve and spectra are shown in all panels as a reference (dotted blue lines).