Fig. 3. m⁶A is critical for ILC2-mediated antihelminth immunity.

a, Quantification of KLRG1⁺ frequency of different cell populations in the small intestine of B6 mice as assessed by flow cytometry. b, Bioanalyzer measurement of the reverse transcripts of mRNAs pulled down by anti-m⁶A antibody in intestinal ILC2s of Mettl3^fl/fl and Mettl3^ΔILC2 mice; IP, immunoprecipitation; FU, fluorescence units; bp, base pairs. c, Quantification of intestinal ILC2s of Mettl3^fl/fl and Mettl3^ΔILC2 mice by flow cytometry; NS P = 0.4378. d,e, Representative flow cytometry (d) and quantification (e) of ST2⁻KLRG1^hi iILC2s in the lungs of Mettl3^fl/fl and Mettl3^ΔILC2 mice that received 3 d of IL-25 injection; *P = 0.0118 (e). f, Quantification of intestinal KLRG1⁺ ILC2s in mice as in d; ****P < 0.0001. g, RT–qPCR of Il5 and Il13 mRNA in the small intestines of mice as in d; *P = 0.0282 (left); *P = 0.0264 (right). h, Quantification of eggs in feces on day 10, worms in the small intestine on day 14 and iILC2s in the lungs on day 5 after inoculation of 300 N. brasiliensis stage-three larvae in Mettl3^fl/fl and Mettl3^ΔILC2 mice; *P = 0.0101 (left); **P = 0.0013 (middle); **P = 0.0021 (right). i, Quantification of eggs in feces on day 11 and worms in the small intestine on day 15 after N. brasiliensis inoculation in Rag2^−/−Il2rg^−/− recipient mice that received cell transfers of ST2⁻KLRG1^hi iILC2s from IL-25-treated Mettl3^fl/fl or Mettl3^Δ/Δ mice. Cell transfers were performed on the same day as N. brasiliensis inoculation, and the recipient mice also received three doses of tamoxifen every other day from days 1 to 5; ***P = 0.0003 (left); **P = 0.0044 (right). Data were analyzed by unpaired two-tailed t-test; n = 3–5 in a, c and e–i. Data are shown as mean ± s.d. Results are representative of three independent experiments.