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. Author manuscript; available in PMC: 2024 Sep 18.
Published in final edited form as: ACS Appl Bio Mater. 2023 Aug 11;6(9):3912–3918. doi: 10.1021/acsabm.3c00574

Figure 2. Cellular uptake of hairpin-like siRNA-LSNAs.

Figure 2.

(A) Hairpin-like siRNA-LSNAs efficiently enter HUVECs in comparison to linear siRNAs, as measured by flow cytometry. Error bars represent the standard deviation of 3 biological replicates. (B) Confocal microscopy images of Cy5-labeled linear siRNAs and hairpin-like siRNA-LSNAs internalized by HUVECs (blue: DAPI stain indicating nucleus, red: Cy5 indicating siRNA), scale bar: 10 μm. Statistical analysis was performed using an unpaired t-test, where “****” represents a P value of <0.0001.