Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jan 5;14:1297367. doi: 10.3389/fgene.2023.1297367

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2024 Guerra, Trerotola, Relli, Lattanzio, Boujnah, Travali, Moschella, Todaro, Pierdomenico, Di Pietro, Tinari and Alberti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Flow cytometry analysis of NHP cDNA transfectants with the indicated anti-Trop-2 mAbs. Transient transfectants of marmoset TROP2 cDNA were generated in HEK-293 cells. Human, baboon and rhesus/cynomolgus monkey TROP2 cDNA were stably transfected in COS-7 cells, following G418-selection and flow-cytometry sorting of expressing population. (A) Cells transfected with the human Trop-2 cDNA. (B) Cells transfected with the rhesus/cynomolgus monkey Trop-2 cDNA (C) Cells transfected with the baboon Trop-2 cDNA. (D) HEK-293 cells transiently transfected with the marmoset Trop-2 cDNA. Efficient binding of 2EF/Hu2EF-Alexa488 (red profiles) and 2G10/Hu2G10-Alexa488 (blue profiles) was found to all NHP Trop-2 transfectants. The Alexa488-tagged AbT16 anti-Trop-2 mAb (green profiles) was used as benchmark. (D) An irrelevant mAb was used as negative control (gray profile); (A) staining using the AF650 anti-Trop-2 goat pAb was used as a positive control (gray profile). Mock transfected cells are in magenta. Unstained cells are in black. Autofluorescence compensation was used in all analyses (Alberti et al., 1987).