Figure 3.

Crystallographic snapshots of the T130K ANT4’ reaction coordinate. A. The T130K ANT4’ neomycin complex active site. The SHB formed between the Glu52 and the neomycin 3’ OH is represented as a red dashed line. B. The ternary (neomycin-AMPCPP) ANT4’ active site. C. The product state (adenylated neomycin) ANT4’ active site. T130K ANT4’ is shown with cyan ribbon representation with yellow carbon atoms for neomycin and magenta carbon atoms for AMPCPP or AMP. Select hydrogen bonds in the active site are represented as black dashed lines. The complete hydrogen bonding interactions are diagramed in Figure S3.