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. Author manuscript; available in PMC: 2024 Jan 19.
Published in final edited form as: ACS Catal. 2020 Feb 18;10(6):3548–3555. doi: 10.1021/acscatal.9b05201

Figure 4.

Figure 4.

Crystallographic snapshots of the T130K/E52D ANT4’ reaction coordinate. A. Superposition of the T130K/E52D ANT4’ neomycin bound active site with the T130K ANT4’ neomycin complex. The 3.2 Â hydrogen bond to the neomycin 3’ OH is represented as a red dashed line. B. Superposition of the T130K/E52D ANT4’ ternary complex (neomycin and AMPCPP) active site with the T130K ANT4’ ternary complex. C. Superposition of the T130K/E52D ANT4’ product state (adenylated neomycin) active site with T130K ANT4’ product complex. The T130K/E52D ANT4’ is shown with magenta ribbon representation with yellow carbon atoms for neomycin and magenta carbon atoms for AMPCPP or AMP. T130K ANT4’ is shown with all gray carbon atoms. Select hydrogen bonds in the active site are represented as black dashed lines. The complete hydrogen bonding interactions are diagramed in Figure S4.