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ITC binding parameters of ANT4’
| Enzyme | Ligand | N | Kd (μM) |
|---|---|---|---|
| T130K | neomycin | 0.9 ± 0.01 | 0.34 ± 0.03 |
| T130K (No Mg2+) | neomycin | 1.0 ± 0.01 | 0.07 ± 0.01 |
| T130K/E52D | neomycin | 0.8 ± 0.01 | 0.87 ± 0.08 |
| T130K/E52D (No Mg2+) | neomycin | 1.0 ± 0.02 | 0.30 ± 0.10 |
| T130K/AMPCPP | neomycin | 0.7 ± 0.004 | 0.13 ± 0.02 |
| T130K/E52D/AMPCPP | neomycin | 0.7 ± 0.001 | 0.12 ± 0.03 |
