Table 4.
Thermal profile of the ERIC-PCR method.
Stage | Temperature [°C] | Time | ||
---|---|---|---|---|
Preliminary denaturation | 95 | 7 min. | ||
Denaturation | Number of cycles | 90 | 30 CYCLES | 30 sec. |
Annealing | 52 | 1 min. | ||
Synthesis | 65 | 8 min. | ||
Final polymerization | 65 | 16 min. |