Exosomal ENO2 was elevated in the DLBCL and DLBCL-derived exosomes regulated macrophages polarization. A-C. TEM images and NTA showed the structures and sizes of exosomes. D. Serum exosomal ENO2 was elevated in the sera of DLBCL patients and related to the progression of DLBCL. E. ENO2 protein levels were higher in both cellular and exosomes of DLBCL cells than that of B-lymphocytes from NCs. F-G. Confocal microscope images showed that THP-1 macrophages uptake exosomes from DLBCL cells. Exosomes (green), F-actin (red), and Nuclei (blue). H. mRNA levels of IL-10, TGF-β, IL-12p40, and TNF-α in THP-1 macrophages. I. IL-10 and IL-12p40 levels in supernatant of THP-1 macrophages. J. Proportion of CD68+CD206+THP-1 macrophages and quantification analysis. Data were shown as the mean±SD. *p<0.05, **p<0.01, ***p<0.001.