TABLE 2.

Box–Behnken design matrix and observed responses: production and productivity of diacetylated lactonic C18:1 and yeast growth.

Run	Combination	$X_1:glucose$	$X_2:urea$	$X_3:time$	Diacetylated lactonic C18:1 production		Diacetylated lactonic C18:1 productivity	Yeast growth
		(Ratio)	(Ratio)	(h)	(Total g)	(g g-1 DMi)	(g L-1 h-1)	(Total CFU)
1	+++	200	2	240	0.624	0.054	0.014	4.57 × 1010
2	-++	50	2	240	0.408	0.040	0.009	3.90 × 1010
3	-0-	50	1	96	0.396	0.039	0.023	2.53 × 1010
4	+00	200	1	168	0.736	0.062	0.024	3.36 × 1010
5	0--	125	0	96	0.242	0.022	0.014	5.76 × 1010
6	+0+	200	1	240	0.866	0.073	0.02	3.45 × 1010
7	0-+	125	0	240	0.735	0.067	0.017	3.94 × 1010
8	--+	50	0	240	0.44	0.043	0.01	4.82 × 1010
9	000a	125	1	168	0.771	0.070	0.025	2.24 × 1010
10	0–0	125	0	168	0.45	0.041	0.015	3.22 × 1010
11	+0-	200	1	96	0.534	0.045	0.031	4.08 × 1010
12	+--	200	0	96	0.333	0.028	0.019	3.97 × 1010
13	++-	200	2	96	0.597	0.050	0.035	4.59 × 1010
14	-+0	50	2	168	0.409	0.040	0.014	2.08 × 1010
15	---	50	0	96	0.329	0.032	0.019	5.45 × 1010
16	−0+	50	1	240	0.438	0.043	0.010	2.32 × 1010
17	+-+	200	0	240	0.699	0.059	0.016	4.37 × 1010
18	000b	125	1	168	0.73	0.067	0.024	5.48 × 1010
19	000c	125	1	168	0.742	0.068	0.025	5.31 × 1010
20	0 + 0	125	2	168	0.531	0.048	0.018	7.93 × 1010
21	00+a	125	1	240	0.763	0.070	0.018	4.39 × 1010
22	++0	200	2	168	0.626	0.053	0.021	3.78 × 1010
23	- + -	50	2	96	0.311	0.030	0.018	3.07 × 1010
24	--0	50	0	168	0.337	0.033	0.011	2.98 × 1010
25	00-a	125	1	96	0.591	0.054	0.034	3.59 × 1010
26	−00	50	1	168	0.338	0.033	0.011	2.50 × 1010
27	00-b	125	1	96	0.587	0.053	0.034	2.78 × 1010
28	00-c	125	1	96	0.537	0.035	0.031	4.99 × 1010
29	0+-	125	2	96	0.529	0.048	0.031	2.58 × 1010
30	+-0	200	0	168	0.685	0.058	0.023	6.53 × 1010
31	00 + b	125	1	240	0.617	0.056	0.014	8.53 × 1010
32	00 + c	125	1	240	0.838	0.076	0.019	5.70 × 1010
33	0++	125	2	240	0.528	0.048	0.012	4.57 × 1010

^a DM, initial dry matter; ^{, b, c} are biological replicates at the central point for the corresponding fermentation time.