Fig. 2.
Tbet -/- attenuates CD4+ T cell infiltration and alters T cell effector subsets. A At 8–12 weeks, Tbet -/- or WT controls were stereotactically injected with Olig001-SYN into the dorsal lateral striatum. After 4 weeks, tissue was harvested to assess for neuroinflammation. B Flow cytometry dot plots of CD8+ (CD45+ , CD11b−, TCRb+ , CD8 +) and CD4+ T cells (CD45+ , CD11b−, TCRb+ , CD4 +). Below the dot plots are the cell counts of CD4+ and CD8+ T cells in the striatum in the presence of α-syn pathology within oligodendrocytes. Mean values ± SEM are plotted ± , non-parametric Wilcoxon, *p < 0.05, **p < 0.01. C Representative immunohistochemistry images of pSer129 (green) and CD4+ (red) in the dorsolateral striatum of Tbet -/- mice and WT controls. Scale bars are at 50uM. D Flow cytometry graphs and their corresponding quantification for the following CD4+ T cell subsets: Th1, Th17, Treg, Th2. Mean values ± SEM are plotted, non-parametric Wilcoxon test, ns = no significance, *p < 0.05, ***p < 0.001. For immunohistochemistry experiments, n = 3 per group. For flow cytometry experiments n = 5 (two mouse striatum tissues pooled per n) per group