Fig. 3.

Tbet -/- attenuates Olig001-SYN mediated demyelination. A Both male and female Tbet -/- mice and WT controls were transduced with Olig001-SYN at 8–12 weeks old. 4 weeks post transduction, tissue was collected and stained with luxol fast blue to determine demyelination in the striatum and corpus collosum. B Representative luxol fast blue images of WT and Tbet -/- where myelinated areas are stained in blue. Bottom left boxes are zoomed areas of demyelination that is highlighted in the red boxes. (C) Quantification of the myelin fold change between the ispi- and contralateral sides of the striatum and corpus collosum in WT and Tbet -/- mice. Mean values ± SEM are plotted, non-parametric Wilcoxon test, *p < 0.05. For immunohistochemistry experiments, n = 3 per group; each data point represents a mouse