Table 4.
Variable importance of the RF models
| Men vs women with CHD model | Importance | CHD men vs non-CVD men model | Importance | CHD women vs non-CVD women model | Importance | ||||
|---|---|---|---|---|---|---|---|---|---|
| g_UBA1819 (Ruminococcaceae) | ♀ | 100.00 | o_Peptostreptococcales_Tissierellales | ♀ | 100.00 | f_Coriobacteriaceae | ♂ | 100.00 | |
| g_Ruminococcus | 83.28 | g_Blautia | 89.11 | c_Negativicutes | ♂ | 80.17 | |||
| g_Bilophila | ♀ | 67.04 | c_Negativicutes | ♀ | 88.56 | o_Peptostreptococcales_Tissierellales | ♂ | 77.78 | |
| g_Coprobacter | 50.15 | f_Coriobacteriaceae | ♀ | 80.76 | f_UCG_010 (Oscillospirales) | 73.80 | |||
| g_Barnesiella | 44.85 | f_Streptococcaceae | ♀ | 76.89 | g_UCG_010 | 68.48 | |||
| g_Alistipes | 43.62 | g_Collinsella | ♀ | 68.36 | g_Collinsella | ♂ | 58.08 | ||
| g_Phascolarctobacterium | ♀ | 41.33 | o_Lactobacillales | ♀ | 67.19 | Ruminococcaceae incertae sedis | 57.79 | ||
| g_Paraprevotella | 38.80 | g_Streptococcus | ♀ | 60.19 | g_Ruminococcus | ♂ | 41.54 | ||
| f_Ruminococcaceae | 37.38 | g_Ruminococcus | ♀ | 57.57 | c_Coriobacteriia | ♂ | 37.40 | ||
| f_Barnesiellaceae | ♂ | 37.28 | g_Subdoligranulum | 54.89 | f_Streptococcaceae | ♂ | 37.17 | ||
| g_Eubacterium_siraeum_group | 36.53 | c_Bacteroidia | 54.28 | o_Coriobacteriales | 35.78 | ||||
| g_Subdoligranulum | ♂ | 36.22 | f_Sutterellaceae | ♀ | 53.39 | o_Lactobacillales | ♂ | 35.33 | |
| g_Parabacteroides | 34.08 | g_UCG_002 (Oscillospiraceae) | 48.34 | p_Actinobacteriota | 32.72 | ||||
| o_Oscillospirales | 33.61 | o_Burkholderiales | ♀ | 47.15 | g_Streptococcus | ♂ | 31.54 | ||
| f_Anaerovoracaceae | 32.19 | f_Oscillospiraceae | 45.45 | o_Burkholderiales | ♂ | 28.56 | |||
| f_Tannerellaceae | 31.97 | p_Bacteroidota | 44.97 | g_UBA1819 (Ruminococcaceae) | 28.23 | ||||
| f_Prevotellaceae | 31.86 | p_Firmicutes | 44.59 | f_Butyricicoccaceae | 27.22 | ||||
| g_Butyricimonas | 31.73 | o_Bacteroidales | 42.07 | g_Bilophila | 27.11 | ||||
| Ruminococcaceae incertae sedis | ♀ | 31.22 | f_Barnesiellaceae | 41.18 | g_Phascolarctobacterium | 26.92 | |||
| f_Rikenellaceae | 31.17 | c_Coriobacteriia | ♀ | 40.66 | f_Sutterellaceae | ♂ | 26.08 | ||
The sex-specific alterations in gut microbiota related to CHD were selected according to the following criteria: (1) bacterial taxa critical to distinguishing CHD men from non-CVD men or bacterial taxa critical to distinguishing CHD women from non-CVD women (2) bacterial taxa that significantly differentiated CHD men and CHD women. Men vs Women with CHD model: ♂, bacterial taxa that significantly differentiated CHD men and CHD women, which was also present among the main important variables on the CHD men vs non-CVD men model (in bold). ♂ bacterial taxa that significantly differentiated CHD men and CHD women, which was also present among the main important variables on the model CHD women vs non-CVD women model (in bold). CHD men vs non-CVD men model: bold, specific taxa in men model (these taxa did not appear in the CHD women vs non-CVD women model, and they are therefore specific to distinguishing CHD men from non-CVD men). ♂, taxa also present among the main important variables on the CHD women vs non-CVD women model. CHD women vs non-CVD women model: bold, specific taxa in women model (these taxa did not appear in the CHD men vs non-CVD men model, and they are therefore specific to distinguishing CHD women from non-CVD women). ♂, taxa also present among the main important variables on the CHD men vs non-CVD men model