Table 2.
Residues phosphorylated in HSF1 from heat-treated cells.
| Peptide | N | Phospho-residue | Dig. |
| VEEApSPGRPpSSVDTLLpSPTALIDSILR | 4 | 314, 319, 326 | Trp. |
| VKEEPPpSPPQpSPR | 2 | 303, 307 | Trp. |
| GHTDTEGRPPpSPPPTSTPEK | 3 | 363 | Trp. |
| VVHIEQGGLVKPERDDTEFQHPCFLR | 1 | (97) | Trp. |
| GHTDTEGRPPpSPPPTSTPEK* | 2 | 363 | Trp. |
| VKEEPPpSPPQpSPR* | 2 | 303, 307 | Trp. |
| QFpSLEHVHGSGPY* | 1 | 230 | Trp. |
| VEEApSPGRPpSSVDTLLpSPTALIDSILR* | 3 | 314, 319, 326 | Trp, Chytrp. |
| VEEASPGRPSSVDTLLpSPTALIDSILR | 2 | 326 | Trp, Chytrp. |
| VEEApSPGRPSSVDTLLpSPTALIDSILR | 2 | 314, 326 | Trp, Chytrp. |
| VKEEPPpSPPQSPR | 1 | 303 | Trp, Chytrp. |
| GHTDTEGRPPpSPPPTSTPEK | 2 | 363 | Trp, Chytrp. |
| KVTpSVSTLKS | 1 | 121 | Trp, Chytrp. |
| PLSSpSPLVR | 1 | 292 | Trp, Chytrp. |
| pSLEHVHGSGPY | 1 | 230 | Trp, Chytrp. |
| DARGHTDTEGRPPpSPPPTSTPEKCLSVACL | 2 | 363 | AspN |
| DERPLSSpSPLVRVK | 2 | 292 | AspN |
| DLFpSPSVTVP | 1 | 419 | AspN |
| DSSLASIQELLpSPQEPPRPPEAENSSP | 1 | 444 | AspN |
| DTLLpSPTALI | 1 | 326 | AspN |
| PASVTALTDARGHTDTEGRPPpSPPPTSTPE | 1 | 363 | Glu-C |
| LLpSPQEPPRPPEAEN | 1 | 444 | Glu-C |
| SEPAPApSVTALTDARGHTDTE | 1 | 344 | Glu-C |
| ApSPGRPSSVDTLLpSPTALID | 2 | 314, 326 | Glu-C |
| LFpSPSVTVPD | 1 | 419 | Glu-C |
N: Unambiguously identified phosphoresidues are bolded and are preceded by p. Where identification was ambiguous, candidate residues are underlined. Dig.: proteinase used for digestion of HSF1. *Radiolabeled peptide. Trp: trypsin; Chytrp: chymotrypsin. For experimental details relating to analysis of HSF1 phosphopeptides and identification of phosphorylated residues see Methods.