Figure 3.

Relationship of fluorescence and motility. After migration on fluorescent beads, single cell fluorescence of T98G (A) and U118MG cells (B) is linearly related to area of fluorescent microspheres cleared by the cell. Fluorescence intensity of individual migrating cells was obtained by tracing each cell outline and measuring total fluorescent signal. Area cleared by each cell was measured by outlining cleared area. Images were collected using a 10X phase objective, 0.3 NA, and Endow GFP filter cube, and captured with a 16 bit CCD camera (Cascade 650, Roper Inc.).