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. 2005 Mar 4;6:5. doi: 10.1186/1471-2199-6-5

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Copyright © 2005 Kucknoor et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunoblots with specific mAb 12G4 detecting AP65 immunoprecipitated from extracts of total trichomonad protein preparations (A) and of proteins after the ligand assay (B) derived from purified hydrogenosomes (lanes 1 through 3) and membrane fractions (lanes 4 through 6). AP65 was immunoprecipitated with mAb from protein extracts from hydrogenosomes and membrane fractions of T. foetus (Tf; lanes 1 and 4), T. vaginalis (Tv; lanes 2 and 5), and transfected T. foetus (Tf-pBS-ap65-neo; lanes 3 and 6). Immunoprecipitated AP65 was prepared as described in the Experimental design section and used in a ligand assay to monitor the amount of AP65 adhesin bound to MS-74 VECs as shown above in Figure 3.