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. 2024 Jan 19;19(1):e0297031. doi: 10.1371/journal.pone.0297031

Fig 1. POA protects from PA injury.

Fig 1

(A) BV-2 cells were pretreated with inhibitors or vehicles for one hour, followed by treatment with BSA for 24 h for cell viability assay. (B) BV-2 cells were pretreated with inhibitors or vehicles for one hour, followed by treatment with PA for 24 h for cell viability assay. (C) Effect of POA on BV-2 cell viability at different concentrations. (D) Effect of different POA:BSA ratios on BV-2 cell viability. (E) PA induced effect of POA on LDH release from BV-2 cells. (F-H) PI staining (red) of cells treated with BSA, 200 μM PA, 200 μM PA+200 μM POA for four hours. (I-K) PI staining merged with DAPI (blue). Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Scale bar: 100 μm.