Table 1.

Theoretical patterns of restriction digestion of short 16S-23S intergenic spacer in different species of Lactobacillus

	SphI	NcoI	NheI	SspI	SfuI	EcoRV	DraI	VspI	HincII	EcoRI	HindIII
Lact. delbrueckii	-a	+a	-	-	+	-	-	-	-	-	-
Lact. amylolyticus	-	+	-	-	+	-	-	-	-	-	+
Lact. acidophilus	-	+	-	-	-	-	-	-	-	+	+
Lact. amylovorus	-	+	-	-	-	-	-	-	-	+	+
Lact. helveticus	-	+	-	-	-	-	-	-	-	+	+
Lact. crispatus	-	+	-	-	-	+	-	-	-	+	+
Lact. perolens	-	-	-	-	-	+	-	-	-	-	+
Lact. gasseri	-	-	-	-	-	+	-	-	-	-	-
Lact. johnsonii	-	-	-	-	-	+	-	-	-	-	-
Lact. jensenii	-	-	-	-	-	+	-	-	-	-	-
Lact. reuterii	+	+	-	-	-	-	-	+	-	-	-
Lact. vaginalis	+	+	-	-	-	-	-	++b	-	-	+
Lact. brevis	+	-	-	-	-	-	-	+	-	-	+
Lact. sakei	+	-	-	-	-	-	++	-	-	-	+
Lact. casei	-	-	-	-	-	+	+	+	-	-	+
Lact. rhamnosus	-	-	-	-	-	+	+	-	-	-	+
Lact. salivarius	-	-	-	+	-	-	++	+	+	-	-
Lact. ruminis	-	-	-	+	+	-	+	-	-	-	-
Lact. plantarum a	+	-	-	+	-c	-	-	+	+	-	-
Lact. plantarum b	+	-	-	+	+c	-	-	+	+	-	-
Lact. fermentum	+	-	-	-	-	-	-	+	-	-	-

^aPlus and minus signals represent positive and negative restriction digestion, respectively

^bTwo or more restriction sites found into spacer region

^cPolymorphic site present in some strains of Lact. plantarum and absent in others