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. 2024 Jan 19;10(3):eadg1222. doi: 10.1126/sciadv.adg1222

Fig. 4. Metabolic maturity of iPSC-aCMs.

Fig. 4.

(A) Immunostaining for mitochondria via MitoView and sarcomeres via α-actinin showed that while the iPSC-aCM mitochondria were randomly distributed in random monoculture (RM), they were linear and aligned with the sarcomeres in PC. Inset images are magnified regions as indicated by the dashed rectangles in the corresponding larger image. (B) TEM images of iPSC-aCMs in RM and PC, with the latter containing elongated mitochondria (*) and aligned sarcomeres (arrow). (C) Seahorse analysis measuring OCR of iPSC-aCMs from RM and PC; additional parameters were calculated from this data. PC showed a significant increase in spare respiratory capacity, ATP production, max respiration, and basal respiration compared to RM (PC: n = 20 wells; RM: n = 12 wells). ***P < 0.001 and ****P < 0.0001. FCCP, carbonyl cyanide (trifluoromethoxy) phenylhydrazone; anti-A/Rot, antimycin A/rotenone; DAPI, 4′,6-diamidino-2-phenylindole. (A) Scale bar, 50 µm (10 µm for inset images) and (B) Scale bar, 1 µm.