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. 2024 Jan 19;10(3):eadg1222. doi: 10.1126/sciadv.adg1222

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Fig. 6. — (A) Verification of siRNA-mediated EFNB1 knockdown via gene expression analysis in ACFs 7 days following transfection (n = 6). Collagen 1a1 (COL1A1) was used an untargeted control ACF gene. Knocking down EFNB1 in ACFs before coculture (B) lowers speed of iPSC-aCM contraction movement (n = 8) and (C) disrupts sarcomere organization in iPSC-aCMs as visualized via immunostaining of cTnT and α-actinin. (D) Verification of siRNA-mediated Cx40 (GJA5) knockdown via gene expression analysis in ACFs 7 days following transfection (n = 6). (E) Knocking down GJA5 in ACFs before coculture lowers speed of iPSC-aCM contraction movement (n = 8). Scale bar, 20 µm.