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[Preprint]. 2024 Jan 5:2024.01.04.574239. [Version 1] doi: 10.1101/2024.01.04.574239

Figure 3. Spatial analysis reveals cell cycle differences in different retinal vascular zones.

Figure 3.

(A) A representative ERG (white)/IB4 (blue) labeled P6 retina image with vascular zones delineated for endothelial cell cycle analysis. Labels to right. Scale bar, 200 μm. (B) Quantification of % mVenus−, mCherry+ (S phase), ERG+ endothelial cells across vascular zones. **** p< 0.0001, *** p< 0.001, ** p< 0.01 by one-way ANOVA & Sidak’s multiple comparisons test. (C-D) Representative images (scale bar, 100μm) (C) and quantification (D) of endothelial cells (ERG+) labeled mVenus+, mCherry− (G1/G0), green arrowheads; mVenus−, mCherry+ (S), red arrowheads; and mVenus+, mCherry+ (G2M), yellow arrowheads; in retinal vascular zones. (E-G) Comparison of AFC and Tip cells. (E) Quantification of % mVenus+, mCherry+, ERG+ (G2) endothelial cells, (F) ratio of endothelial cells in G2 to S, (G) ratio of endothelial cells in G2 to G1/G0 in AFC vs. tip cells. * p< 0.05, *** p< 0.001 by paired t test. n = 4 pups for all quantifications.