Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Mar 24;6:20. doi: 10.1186/1471-2202-6-20

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005 Alonso et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Cell proliferation mostly concerns SON capillary endothelial cells. BrdU was administrated to osmotically stimulated rats drinking 2% saline during 6 days, and the animals were fixed 5 hours after the BrdU administration. A, C: Stack confocal images (10 μm-thick) of sections labeled for endothelial cell markers. EBA immunostaining is associated with vessels located within the SON and the surrounding regions (A), whereas nestin immunostaining is associated with SON vessels, but only faintly labels those vessels located in the surrounding regions (C). B, D: Merged confocal images of sections double labeled for BrdU and for one of the two the endothelial cell markers. Throughout the SON, BrdU-labeled nuclei are frequently associated with vessel structures labeled for EBA (arrows in B) or nestin (arrows in D). EBA: endothelial brain antigen; OC: optic chiasma. Scale bars: A, C = 100 μm; B, D = 25 μm.